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. 2025 May 15;26(10):4744. doi: 10.3390/ijms26104744

  • HPLC:

 1100 Series HPLC System with MWD (UV/VIS Detector), Agilent Technologies;

  • Separation Mode:

 Isocratic (Reversed-Phase Separation);

  • Column:

 Water XTERRA RP-18 (4.6 × 250 mm, 5 µm);

  • Mobile Phase:

 Solvent A: 25 mM Potassium Phosphate Monobasic buffer 2.9; Solvent B: 100% can;

  • Solvent Strength:

 (70:30 v/v) Buffer: Can;

  • Absorbance:

 249 nm;

  • Flow Rate:

 1.0 mL/min;

  • Injection Volume:

 15 µL;

  • Column Temperature:

 Ambient;

  • Run Time:

 18 min;