Figure 4.

This figure illustrates a simplified dual-index barcoding approach, facilitating the simultaneous pooling of various sample types, including cDNA libraries for FOX hunting mutagenesis, gRNA for CRISPR/Cas9-mediated mutagenesis, and genomic DNA for identifying transposon or T-DNA insertion sites. This innovative design enhances the efficiency of next-generation sequencing (NGS) by allowing for the multiplexing of samples, thereby reducing costs and improving the throughput in genotyping. The color is to indicate the unique combinatorial barcoding method.