FIG. 2.

HLA restriction of NS3/4-specific CD4⁺ T-cell clones. NS3/4-specific CD4⁺ T-cell clones were incubated with partially matched homozygous lymphoblastoid cell lines carrying one of the patient's HLA-DR alleles, in the presence of recombinant NS3/4 protein (black bars) or medium control (empty bars). After 16 h activation of specific CD4⁺ T-cell clones was determined by fluorescence-activated cell sorting for CD25 expression and shown as mean fluorescence intensity (MFI).