TABLE 3.
Mapping of the minimal, stimulatory sequence within CD4+ T-cell epitopes
| No. | aa | Patient(s) | Source | Minimal/optimal epitopea | HLA restriction |
|---|---|---|---|---|---|
| p22 | 1388-1407 | 14, 19 | Clones | GRHLIFCHSKRKCDELATKL | DRB1*1501/DRB5*0501 |
| 14 | PBMC | GRHLIFCHSKR | DRB1*1502/*1104 | ||
| p24 | 1406-1425 | 12 | Clone | KLVAMGINAVAYYRGLDVSV | DRB1*1501 |
| PBMC | |||||
| p37 | 1535-1554 | 2 | Clone | TTVRLRAYMNTPGLPVCQDH | DRB1*0701 |
| 2 | PBMC | AYMNTPGLPV | DRB1*0101/*0701 | ||
| 14 | PBMC | LRAYMNTPGLPV | DRB1*1502/*1104 | ||
| p42 | 1585-1604 | 7 | Clone | YLVAYQATVCARAQAPPPSW | DRB1*1501 |
| 3 | PBMC | YLVAYQATVC | DRB1*0301/*1501 | ||
| 2 | PBMC | NLPYLVAYQATVCAR | DRB1*0101/*0701 | ||
| p49 | 1655-1674 | 21 | Clone | VVTSTWVLVGGVLAALAAYC | DRB1*0101/*1301 |
| 2 | PBMC | TSTWVLVGGVLAA | DRB1*0101/*0701 | ||
| p52 | 1685-1704 | 6 | Clone | RVVLSGKPAIIPDREVLYRE | DRB1*0301 |
| 3 | PBMC | IIPDREVLYR | DRB1*0301/*1501 | ||
| p60 | 1765-1784 | 13 | Clone | MWNFISGIQYLAGLSTLPGN | DRB1*1104 |
| 14 | PBMC | YLAGLSTLPG | DRB1*1502/*1104 | ||
| p61 | 1775-1794 | 22 | Clone | LAGLSTLPGNPAIASLMAFT | DRB1*0402/*1101 |
| p62 | 1785-1804 | 13 | Clone | PAIASLMAFTAAVTSPLTTS | DRB1*0102 |
| 14 | PBMC | PAIASLMAFTA | DRB1*1502/*1104 | ||
| p64 | 1805-1824 | 2, 12, 13 | Clones | QTLLFNILGGWVAAQLAAPG | DRB1*0101, *0102 |
| 21 | PBMC | FNILGGWVAAQL | DRB1*0101/*1301 | ||
| 2 | PBMC | FNILGGWVA | DRB1*0101/*0701 | ||
| p71 | 1875-1894 | 12 | Clone | STEDLVNLLPAILSPGALVV | DRB1*0101 |
| 2 | PBMC | NLLPAIL | DRB1*0101, *0701 |
a
Boldface amino acids are required for optimal T-cell stimulation; the underlined amino acids represent the minimal epitope that induces significant T-cell stimulation.