FIG. 2.

Induction of TLR3 surface protein expression is dose responsive and serotype independent and requires actively replicating virus. (A) Cells were infected for 1 h with three doses of RV-16 at MOIs of 1, 0.5, and 0.2. Cells were harvested for flow cytometry 24 h postinfection. (B) Cells were infected for 1 h with RV-16 (MOI of 1), RV-1B (MOI of 1), RV-9 (MOI of 0.5), UV-inactivated RV-16 (MOI of 1), or the same volume of filtered RV-9. Cells were harvested for flow cytometry 24 h postinfection. Data are presented as MFI minus the MFI of isotype control stained cells (n = 3). Data are considered significantly different between infected and uninfected control cells at P < 0.05 (*) and P < 0.01 (**). ns, data not significantly different at P > 0.05.