FIG. 6.

Cytokine antibody array and ELISA. (A) Cytokine antibody array. BJAB cells (10⁷) were electroporated with 20 mg each of pcDEF, pcDEF/K1, pcDEF/K1 Y₁F, pcDEF/K1 Y₃F, or pcDEF/K1 TYF. Live cells were separated by Ficoll-Hypaque centrifugation at 6 h posttransfection and cultured for additional 30 h. The supernatants were collected, spun down to remove cell debris, and subjected to a cytokine antibody array. The antibody array was carried out in accordance with the manufacturer's recommendations using a cytokine array membrane (Panomics, Redwood City, CA), which contains 42 cytokines and chemokines and six controls. (B) Values from the cytokine array membrane were evaluated by densitometry after subtraction of the background signal and normalized by the control signal. (C) MDC and IL-10 ELISA. Supernatants were prepared as described above. ELISA (BD PharMingen) was carried out in accordance with the manufacturer's recommendations. Vec, vector.