TABLE 2.
Kinetic and affinity data for the interactions of VCP and the CCP 1-3 mutant with C3b and C4ba
| Ligand | Analyte | kd(1/s)/ka(1/Ms) | SE (kd/ka) | KDa (μM) | χ2 |
|---|---|---|---|---|---|
| C3b | VCP | 1.57/9.07 × 105 | 1.56 × 10−1/9.58 × 104 | 1.73b | 0.327 |
| C3b | VCP | NA | NA | 1.94c | 0.521 |
| C4b | VCP | 0.309/1.61 × 106 | 6.56 × 10−3/3.52 × 104 | 0.193b | 0.775 |
| C4b | VCP | NA | NA | 0.185c | 1.52 |
| C3b | CCP 1-3 | 0.13/2.08 × 103 | 9.59 × 10−3/269 | 62.4b | 0.131 |
| C3b | CCP 1-3 | NA | NA | 68.4c | 0.0633 |
| C4b | CCP 1-3 | 0.363/8.27 × 103 | 0.0102/266 | 43.9b | 0.236 |
| C4b | CCP 1-3 | NA | NA | 42.3c | 0.109 |
a
NA, not applicable; ka, association rate constant; kd, dissociation rate constant; KD, equilibrium rate constant; SE, standard error.
b
Data were calculated by global fitting to a 1:1 Langmuir binding model (BIAevaluation 4.1).
c
Data were calculated by steady-state analysis (BIAevaluation 4.1).