TABLE 2.
Summary of sedimentation analysis of in vitro-synthesized VP22 and deletion variantsa
Protein (size, kDa) | Peak | S | Size (kDa)
|
|
---|---|---|---|---|
150 mM NaCl | 500 mM NaCl | |||
VP22 (32.2) | Major | 5.4-6.9 | 101-146 | 34-79 |
VP22.60-301 (25.3) | Minor | ∼5.8 | ∼113 | 34 |
Major | 4.3 | 68 | 34 | |
VP22.1-267 (28.6) | Minor | ∼6.6 | ∼134 | 40 |
Major | 5.1 | 90 |
Summary of the sedimentation profiles of in vitro-synthesized VP22 and deletion variants. Sedimentation was performed at either 150 mM or 500 mM NaCl. The presence of minor species is discussed in the text. Only size is given for the results at 500 mM NaCl. The predicted size for each species as calculated from the linear sequence of amino acids is given below each variant in the left-hand column.