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. 2005 Oct;79(19):12602–12607. doi: 10.1128/JVI.79.19.12602-12607.2005

FIG. 3.

FIG. 3.

Construction of an ambisense BUNV model template able to express an additional foreign protein. (A) A cDNA encompassing the entire GFP ORF was inserted at the XhoI site directly downstream of the antigenomic 3′ NTR of templates WT(25/25)-U/A, AG-CB(25/25)-U/G, and AG-CB(25/25)-U/A. (B) Plasmids expressing the antigenomic strand of templates WT(25/25)-U/A-GFP, AG-CB(25/25)-U/G-GFP, and AG-CB(25/25)-U/A-GFP were transfected into vTF7-3-infected BHK-21 cells together with plasmids expressing BUNV S and L support plasmids (+pL) or BUNV S support plasmid alone (−pL). The ability of the antigenomic strands of these templates to express GFP was analyzed using a Nikon Eclipse TE2000-E fluorescence microscope 20 h posttransfection at a magnification of 60×.