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. 2005 Oct;79(19):12148–12163. doi: 10.1128/JVI.79.19.12148-12163.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 4. — Competition ELISA between immune rabbit sera and MAbs for binding to gp120. Shown here are values obtained from competition ELISA in which wild-type gp120- and GDMR gp120 immune sera binding to wild-type gp120 is inhibited in the presence of human MAbs specific to the V3 loop (447-52D, F425-B4e8, and 39F). Percentage of V3 loop antibodies in the total polyclonal sera was determined by percent inhibition of serum binding to wild-type gp120 (as described in Materials and Methods) and is shown on the y axis. The percentages shown on the graph for wild-type gp120 and GDMR gp120 mutant are the averages of values obtained for three rabbits per group.