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. 2005 Oct;79(19):12148–12163. doi: 10.1128/JVI.79.19.12148-12163.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 5. — Competition ELISAs between biotinylated MAbs and immune rabbit sera. The x axis represents the concentration of biotinylated human MAb (μg/ml). The y axis represents the binding of biotinylated MAb detected with streptavidin-alkaline phosphatase at OD₄₀₅. Biotinylated monoclonal antibody binding was inhibited in the presence of a single dilution (1/100) of wild-type gp120- or GDMR gp120 immune sera. The wild-type gp120-immunized rabbit sera (rabbits 7262 to 7264) inhibiting the binding of the respective bio-MAbs are shown as black lines; and the GDMR gp120-immunized rabbit sera (rabbits 7265 to 7267) inhibiting the binding of the respective bio-MAbs are shown as gray lines. (A) Inhibition of bio-b12 or bio-2G12 (top graphs) and bio-b6 or bio-F105 (bottom graphs). (B) Inhibition of bio-48d or bio-17b (in the presence of 2 μg/ml, sCD4) (top graphs) and bio-447-52D or bio-F425-B4e8 (bottom graphs).

FIG. 5. — Competition ELISAs between biotinylated MAbs and immune rabbit sera. The x axis represents the concentration of biotinylated human MAb (μg/ml). The y axis represents the binding of biotinylated MAb detected with streptavidin-alkaline phosphatase at OD₄₀₅. Biotinylated monoclonal antibody binding was inhibited in the presence of a single dilution (1/100) of wild-type gp120- or GDMR gp120 immune sera. The wild-type gp120-immunized rabbit sera (rabbits 7262 to 7264) inhibiting the binding of the respective bio-MAbs are shown as black lines; and the GDMR gp120-immunized rabbit sera (rabbits 7265 to 7267) inhibiting the binding of the respective bio-MAbs are shown as gray lines. (A) Inhibition of bio-b12 or bio-2G12 (top graphs) and bio-b6 or bio-F105 (bottom graphs). (B) Inhibition of bio-48d or bio-17b (in the presence of 2 μg/ml, sCD4) (top graphs) and bio-447-52D or bio-F425-B4e8 (bottom graphs).