FIG. 2.

Growth and genomic structure properties of ΔUL37x1 viruses derived on complementing vMIA-HF and noncomplementing HF cells. (A) Mean viral yields with standard deviations from HF cultures infected for 14 days with three transfection-independent viruses derived on HF or vMIA-HF at an MOI of 0.001. (B) Restriction fragment length polymorphism analysis of EcoRV-digested ΔUL37x1 or Towne-BAC virion DNA recovered from independent transfections of HFs (isolates a, b, and c) and vMIA-HFs (isolates d, e, and f), with two dashes indicating the expected polymorphism due to insertion of the KanMX4 cassette. (C and D) Infected cell and focus formation by ΔUL37x1 and Towne-BAC following transfection of equivalent amounts of BAC DNA into noncomplementing HFs. The mean numbers of single GFP-positive (GFP+) cells (C) and GFP-positive foci (D), monitored by live cell microscopy, are graphed with standard deviations from four independent transfections of ΔUL37x1 or Towne-BAC.