FIG. 1.

Composition of the PRRSV constructs. (A) Design of the PRRSV knockout constructs. Depicted are the point mutations of the initiation codons of ORF2a (KO2a), ORF2b (KO2b), ORF5 (KO5), and ORF6 (KO6); the deletions in ORF3 (KO3) and ORF4 (KO4); and the deletion of ORF2 to ORF7 (R⁻). The names of the constructs and the nucleotide (nt) positions of the mutations are shown on the right. (B) Design of the recombinant SFV constructs. Restriction enzyme sites, their positions in the nucleotide sequence of PRRSV LV (GenBank accession no. M96262), and the names of the constructs providing the genes (pABV) are indicated.