TABLE 2.
Rescue of PRRSV gene knockout mutants with constructs expressing the lacking protein
| Mutant | Complementa | p0 BHK-21b | p1 PAMsb | p2 PAMsb |
|---|---|---|---|---|
| Wild type | — | +++ | +++ | +++ |
| KO2A | — | +++ | − | − |
| KO2A | pCIneo-ORF2a | +++ | + | − |
| KO2A | SFV-ORF2 | +++ | + | − |
| KO2B | — | +++ | − | − |
| KO2B | pCIneo-ORF2b | +++ | + | − |
| KO2B | SFV-ORF2 | +++ | + | − |
a
Expression constructs and recombinant SFV used for in trans complementation of the full-length gene knockout constructs. —, no complement added.
b
Virus replication was analyzed by IPMA at 24 h posttransfection (BHK-21) or postinfection (PAMs) using MAb 122.17 directed against the N protein. The degree of positive staining is indicated by the number of plusses (+ to +++); no staining is indicated by a minus (−).