TABLE 1.
Infectivity, in vitro replicative capacity, and IC50 values of sera and sCD4 against mutant strains lacking glycosylation sites proximal to CD4 contact residuesa
| Viral strain | Infectivity (% of WT) | Growth in tissue culture | IC50 sera (inverse dilution) | IC50 sCD4 (ng/μl) |
|---|---|---|---|---|
| SIV239 | NA | Yes | <20 | 17.8 |
| g3 | 170 | Yes | <20 | 4.0 |
| g11 | 8 | Yes | <20 | 3.6 |
| g16 | 35 | Yes | 15 | 39.0 |
| g19 | 65 | Yes | 20 | 6.0 |
| g20 | 33 | Yes | <20 | 24.0 |
| g22 | 69 | Yes | <20 | 16.0 |
| g23 | 82 | Yes | <20 | 12.0 |
| g3,11 | 14 | Yes | <20 | 3.5 |
| g19,20 | 12 | Yes | <20 | 22.0 |
| g22,23 | 59 | Yes | <20 | 9.0 |
| g14,22,23 | 2 | Yesb | NA | NA |
| g16,22,23 | 0 | No | NA | NA |
| g19,20,22,23 | 33 | Yes | 30 | 32 |
| g3,11,19,20 | 17 | Yes | >20 | 9.4 |
| g16,19,20,22,23 | 0 | No | NA | NA |
| g14,19,20,22,23 | 0 | No | NA | NA |
| g15,19,20,22,23 | 0 | No | NA | NA |
| g11,19,20,22,23 | 2 | No | NA | NA |
a
NA, nonapplicable due to low to no infectivity.
b
Replication was noted by day 32, and sequencing of reverse transcription-PCR products derived from cell-free virus revealed nonsynonymous substitutions that were likely compensatory. The mutants in boldface type are those with enhanced neutralization sensitivity to sCD4.