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. 2025 May 14;16:1555186. doi: 10.3389/fendo.2025.1555186

Table 3A.

Summary of findings on physical changes and hormone levels on GnRHa treatment in TGD adolescents.

Reference Study design/ Population Intervention (duration) Height Sexual characteristics Gonadotropins and sexual steroids Body composition Lipids /glucose
Boogers 2022 (10) R/161
(161 AMAB, 0 AFAB)
GnRHa (2.4 ± 0.8 y)
GAHT
↓ (3m) during GnRH
↓ (adult) High dose E and EE
↓ Bone age during GnRH
N/A N/A N/A N/A
Fisher 2024 (4) P/36
(14 AMAB, 22 AFAB)
GnRHa (3-12 m) ↓ HT centile in AMAB ↓ Tanner stage ↓ FSH/LH and T in AMAB/E in AFAB ↑ BMI in AFAB ↓ HDL in AMAB
=
Klaver 2018 (11) R/192
(71 AMAB, 121 AFAB)
GnRHa
AMAB: 2.1 y (1.0-2.8) AFAB: 1.0 years (0.5-2.9) GAHT
N/A N/A N/A WHR and body composition changed toward the affirmed sex N/A
Klaver 2020 (12) R/192
(71 AMAB, 121 AFAB)
GnRHa AMAB: 2.1 y (1.0-2.8); AFAB: 1.0 y (0.5-2.9)
GAHT
N/A N/A N/A > % of obesity vs controls = Changes in lipids, insulin, HOMA IR, BP and BMI similar to controls
Navabi 2021 (13) R/172
(51 AMAB, 119 AFAB)
GnRHa (N/A) N/A N/A N/A
↑ total body fat % increase in gynoid (%fat), and android (%fat) in AFAB and AMAB was detected
without changes in BMI z score
N/A
Schagen 2016 (14) P/116
(49 AMAB, 67 AFAB)
GnRHa (N/A) ↓ (3m) during GnRH ↓ (3m)
during GnRH
↓ (3m)
during GnRH
↓ Lean body mass % and ↑ fat at 1y N/A
Schagen 2018 (15) P/127
(73 AMAB, 54 AFAB)
GnRHa (2y)
GAHT
N/A N/A ↑ DHEAS / ↑ A N/A N/A
Schulmeister 2022 (16) P/55
(26 AMAB, 29 AFAB)
GnRHa (1y) No differences vs controls in early PS
Lower HV in later Tanner stage PS initiation
N/A N/A N/A N/A
Valentine 2022 (17) R-C/4172
(1407 AMAB, 2766 AFAB)
GnRHa (N/A) GAHT N/A N/A N/A ↑ overweight/obesity ↑ dyslipidemia, liver dysfunction and hypertension
Van de Grift 2020 (18) R/200
(66 AMAB, 134 AFAB)
GnRHa (3y)
GAHT
No difference in FH among early, later, or no GnRHa ↓ during GnRH N/A No difference in BMI SDS among early, later, or no GnRHa N/A
Willemsen 2023 (19) R/146
(0 AMAB, 146 AFAB)
GnRHa (3.1±0.9 y) + Testosteron ↓ HV (during GnRHa) ↑ HV (during GAHT) FH exceeded PAH by 3.0 ± 3.6 cm and MPH by 3.9 ± 6.0 cm N/A N/A N/A N/A

A, androstenedione; AFAB, assigned female at birth; AMAB, assigned male at birth; AST, aspartate aminotransferase; BMI, body mass index; BMIz, BMI z-score; BP, blood pressure; C, cross-sectional; DBP, diastolic blood pressure; E, estrogen; EE, ethinyl estradiol; FSH, follicular hormone; f/up, follow-up; FH, final height; GAHT, gender-affirming hormone therapy; GD, gender dysphoria; GnRHa, gonadotropin-releasing hormone agonist; HbA1c, hemoglobin A1C; HDL, high-density lipoprotein; HOMA-IR, homeostatic model assessment for insulin resistance; HT, height; HTz, height z-score; im, intramuscularly; LBW, lean body weight; LDL, low-density lipoprotein; LH, luteinizing hormone; N/A, not available; NS, not significant; P, prospective; PAH, predicted adult height; pc, percentile; PS, pubertal suppression; R, retrospective; SBP, systolic blood pressure; sc, subcutaneously; T, testosterone; TBF, total body fat; TGD, transgender and gender-diverse; TV, testicular volume; WT, weight; WTz, weight z-score; WHR, waist-hip ratio; y, year(s).

Only studies with moderate-high level quality of evidence are included.Arrow down: reduced; Arrow up: increased.