Table 1.
List of NMR experiments acquired at 700 MHz spectrometer at 298 K and corresponding main parameters used for backbone and side-chain assignments for RuV apo (a) and ADPr bound (b) form.
| Time domain data size (points) | Spectral width/Carrier frequency (ppm) | NS | Delay time (s) | |||||
|---|---|---|---|---|---|---|---|---|
| t1 | t2 | t3 | F1 | F2 | F3 | |||
| (a) RuV apo | ||||||||
| 1H,15N HSQC | 256 | 2048 | 44/120 (15N) | 14/4.7 (1H) | 4 | 1 | ||
| 1H,13C HSQC | 512 | 2048 | 160/80 (13C) | 14/4.7 (1H) | 32 | 1 | ||
| CBCANH | 96 | 40 | 1024 | 72/39 (13C) | 44/117 (15N) | 14/4.7 (1H) | 32 | 1 |
| CBCA(CO)NH | 96 | 40 | 1024 | 72/39 (13C) | 44/117 (15N) | 14/4.7 (1H) | 32 | 1 |
| HNCA | 80 | 40 | 1024 | 42/55 (13C) | 44/117 (15N) | 14/4.7 (1H) | 8 | 1 |
| HN(CO)CA | 80 | 40 | 1024 | 42/55 (13C) | 44/117 (15N) | 14/4.7 (1H) | 16 | 1 |
| HNCO | 64 | 40 | 1024 | 18/175 (13C) | 44/117 (15N) | 14/4.7 (1H) | 8 | 1 |
| HN(CA)CO | 64 | 40 | 1024 | 18/175 (13C) | 44/117 (15N) | 14/4.7 (1H) | 8 | 1 |
| HNHA | 48 | 96 | 1024 | 35/117 (15N) | 14/4.7 (1H) | 14/4.7 (1H) | 16 | 1 |
| HBHA(CBCACO)NH | 112 | 40 | 1024 | 8/4.7 (1H) | 44/117 (15N) | 14/4.7 (1H) | 16 | 1 |
| (H)CCH-TOCSY | 128 | 48 | 1024 | 80/39 (13C) | 80/39 (13C) | 14/4.7 (1H) | 16 | 1 |
| 1H, 15N NOESY | 232 | 48 | 2048 | 14/4.7 (1H) | 40/117 (15N) | 14/4.7 (1H) | 16 | 1 |
| 1H, 13C NOESY aliphatic | 192 | 64 | 1024 | 14/4.7 (1H) | 80/39 (13C) | 14/4.7 (1H) | 8 | 1 |
| 1H, 13C NOESY aromatic | 144 | 32 | 2048 | 14/4.7 (1H) | 39/127 (13C) | 14/4.7 (1H) | 8 | 1 |
| (b) RuV ADPr bound | ||||||||
| 1H,15N HSQC | 256 | 2048 | 44/120 (15N) | 14/4.7 (1H) | 4 | 1 | ||
| 1H,13C HSQC | 512 | 2048 | 160/80 (13C) | 14/4.7 (1H) | 32 | 1 | ||
| CBCANH | 96 | 40 | 1024 | 72/39 (13C) | 44/117 (15N) | 14/4.7 (1H) | 32 | 1 |
| CBCA(CO)NH | 96 | 40 | 1024 | 72/39 (13C) | 44/117 (15N) | 14/4.7 (1H) | 32 | 1 |
| HNCA | 80 | 40 | 1024 | 42/55 (13C) | 44/117 (15N) | 14/4.7 (1H) | 8 | 1 |
| HN(CO)CA | 80 | 40 | 1024 | 42/55 (13C) | 44/117 (15N) | 14/4.7 (1H) | 16 | 1 |
| HNCO | 64 | 40 | 1024 | 18/175 (13C) | 44/117 (15N) | 14/4.7 (1H) | 8 | 1 |
| HN(CA)CO | 64 | 40 | 1024 | 18/175 (13C) | 44/117 (15N) | 14/4.7 (1H) | 8 | 1 |
| HNHA | 48 | 96 | 1024 | 44/120 (15N) | 14/4.7 (1H) | 14/4.7 (1H) | 16 | 1 |
| HBHA(CBCACO)NH | 112 | 40 | 1024 | 8/4.7 (1H) | 44/117 (15N) | 14/4.7 (1H) | 16 | 1 |
| (H)CCH-TOCSY | 128 | 48 | 1024 | 80/39 (13C) | 80/39 (13C) | 14/4.7 (1H) | 16 | 1 |
| 1H, 15N NOESY | 232 | 48 | 2048 | 14/4.7 (1H) | 44/120 (15N) | 14/4.7 (1H) | 8 | 1 |
| 1H, 13C NOESY aliphatic | 192 | 64 | 1024 | 14/4.7 (1H) | 80/39 (13C) | 14/4.7 (1H) | 8 | 1 |
| 1H, 13C NOESY aromatic | 144 | 32 | 2048 | 14/4.7 (1H) | 39/127 (13C) | 14/4.7 (1H) | 8 | 1 |