Fig. 3. Experimental validation of full-color HiLo microscopy.

a1–a4 Pairs of widefield images (left) and HiLo images (right) of mouse lung, kidney, liver, and brain tissue, respectively. b1–b4 Zoomed-in pairs of TRUST images (left) and HiLoTRUST images (right) of the regions indicated in a1–a4. c1–c4 Line profiles of solid white lines regions marked in b1–b4 with min-max normalization, respectively. Note: widefield and HiLo images have been normalized with min-max normalization for better visualization (see Supplementary Figure 4 for HiLo images without normalization).