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Chromatograms of the crude conjugated product (pink lines) and blank (black lines) obtained at column temperatures of (a) 40, (b) 60, (c) 70, and (d) 80 °C. Red triangles indicate shoulder peaks partially separated from the R9-ER(dec)-R peaks. At 40 °C, no elution of R9-ER(dec)-R was observed, although the UV absorption signal showed a slight increase from 7 to 9 min (green triangle).
