Table 2. Plasmids used for expression of kinetochore proteins*.
| Protein | Plasmid | Components | References |
|---|---|---|---|
| Nucleosome | pScKl2 |
K. lactis His6-H2A, K. lactis His6-H2B, Cse4, K. lactis His6-H4 |
Migl et al., 2020 |
| OA | pGH3 | Okp1, Ame1-FLAG | Hamilton et al., 2020 |
| Mif2 | pAZ144 | Mif2-MBP | This study |
| 2D-MIND | pGH62 | FLAG-Nsl1, S240D & S250D Dsn1, Mtw1, Nnf1 | Hamilton et al., 2020 |
| (230-576) 2D-MIND | pEHM4 | FLAG-Nsl1, (230-576) S240D & S250D Dsn1, Mtw1, Nnf1 | Hamilton et al., 2020 |
| Ndc80c | pJT48 Ndc80/Nuf2 |
Spc24-FLAG, Spc25 Ndc80, Nuf2 |
Kudalkar et al., 2015; Wei et al., 2005 |
| Dam1c | pJT44 | Spc34-FLAG, Dad1, Dad2, Dad3, Dad4 Duo1, Dam1, Hsk3, Spc19, Ask1 | Umbreit et al., 2014 |
*
All proteins are from Saccharomyces cerevisiae except as noted.