Table 3. DNA sequences tested for ability to wrap centromeric nucleosomes.
| Name | Sequence* | Wrapped? | Stable duringSEC? |
|---|---|---|---|
| W601† |
ATCGAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAGACAGC
TCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTA ACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATA TATACATCCGAT |
Yes | Yes |
| CEN 3 ‡ |
AAAGCTATTCATTGAAAAAATAGTACAAATAA
GTCACATG
ATGAT
ATTTGATTTTATTATATTTTTAAAAAAAGTAAAAAATAAAAAGT
A
GTTTATTTTTAAAAAATAAAATTTAAAATATTAG TGTATTTGATT T CCGAAAGTTAAAA AAGAAATAGTAAGAAATATATATTT |
Some | No |
| CEN 5.1 |
AAAGCTATTCATTGAAAAAATAGTACAAATAAGTCACATGATCGA GAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAGACAGCTCTAG CACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAACCGC CAAGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATATATAC ATCCGATTATTTGATTTCCGAAAGTTAAAAAAGAAATAGTAAGAA ATATATATTT |
Yes | Yes |
| CEN3-601 (Xiao et al., 2017) |
ATCGAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAGACAGC
TCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTA ATATTAGTGTATTTGATTTCCGAAAGTTAAAAAAGAAATAGTAAG AAATCATCCGAT |
Yes | Yes |
| CCEN† |
ATCGAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAGACAGC TCTAGCACCGCTTAAACAGTTTATTTTTAAAAAATAAAATTTAAA ATATTAGTGTATTTGATTTCCGAAAGTTAAAAAAGAAATAGTAAG AAATCATCCGAT |
Yes | Yes |
| CEN 6.3 |
ATCGAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAGACAGC
TCTAGCACCGCTTAAACAGTTTATTTTTAAAAAATAAAATTTAAA ATATTAGAGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATA TATACATCCGAT |
Yes | ND § |
| CEN 6.4 |
ATCGAGAATCCCGGTGCCGAGGCCGCTCAATTGGTCGTAGACAGC
TCTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTA ACCGCCAAGGGGATTACTCCCTAGTCTCCAGGCACGTGTCAGATA TATACATCCGAT GCCGCC AGTTTATTTTTAAAAAATAAAATTTAA AATATTAG |
Some | ND § |
| CON3 ¶ (Dendooven et al., 2023) |
ATAAGTCACATGGTGCCGAGGCCGCTCAATTGGTCGTAGACAGCT CTAGCACCGCTTAAACGCACGTACGCGCTGTCCCCCGCGTTTTAA TATTAGTGTATTTGATTTCCGAAAGTTAAAAAAGAAATAGTAAGA AATATATATTTCATTGAA |
ND § | ND § |
ND, not determined.
*
W601 DNA in black font; Genomic DNA upstream or downstream of CEN3 in blue font; CDEI in purple font; CDEII in red font; CDEII Mif2 footprint in red font and underlined; CDEIII in purple font and underlined; linker DNA highlighted in bold.
†
These are the DNA sequences used to wrap the nucleosomes tested in the optical trapping assay in this paper.
‡
The construct used by Xiao et al., 2017 included only the 147 bp of CEN3 and none of the upstream or downstream sequences.
§
Not determined.
¶
Shown for comparison only.