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. 2025 Apr 16;417(15):3465–3476. doi: 10.1007/s00216-025-05878-0

Table 3.

Stability of samples up to 14 days after preparation. CV% as calculated from pg observed is calculated from the data corrected by the calibration curve prepared on the day of analysis. CV% as calculated from raw data is not corrected with a calibration curve, but calculated directly from the [peak area cortisol]/[peak area internal standard] results

Samples Average pg cortisol observed CV% as calculated from pg observed CV% as calculated from raw data
Pool A 174 5.0% 2.0%
Pool B 131 4.8% 1.7%
Pool C 156 6.7% 1.4%
Pool D 119 8.6% 3.1%