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. 2025 Apr 16;417(15):3465–3476. doi: 10.1007/s00216-025-05878-0

Table 4.

Cortisol concentration results obtained for hair samples divided into two aliquots, prepared either with methanol extraction of the hair, or with the digestion protocol Standard deviation is provided in brackets

Sample Methanol extraction [pg/mg hair] Enzymatic digestion [pg/mg hair] Ratio (%) methanol/enzymatic digestion
1 2.40 3.36 71%
2 4.15 5.06 82%
3 4.27 5.03 85%
4 4.40 4.94 89%
5 4.73 5.86 81%
6 4.76 5.79 82%
7 12.71 19.13 66%
8 44.31 50.45 88%
Mean (SD) 81% (8%)