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. 2025 May 30;109(1):131. doi: 10.1007/s00253-025-13519-w

Table 2.

Summary of studies that applied micro-aeration for anaerobic removal of pollutants, including key parameters such as substrate, reactor configuration, air/oxygen dosage, and observed effects

Substrate Reactor type Air/pure O2 Pretreatment (Pt)
Direct (D)
Aerationa Effect Reference
Olive mill wastewater Glass flask (batch) Air Pt—continuous (5 or 7 days) 936 L L−1 d−1

Decrease of phenols (78–90%)

Decrease total COD (65%)

González-González and Cuadros (2015)
Synthetic BTEX-contaminated water UASB Air D—intermittent (through feeding line) 0.07, 0.10, and 0.30 L L−1 d−1

Removal of BTEX (> 83%)

Without volatilization

Siqueira et al. ((2018)
Petrochemical wastewater Digestion reactor Air Pt—intermittent (ORP-based) 0.04–0.06 mg L−1

Reduced H2S

Removal of BTEX

Wu et al. (2015)
Petrochemical wastewater Full scale Air Pt—intermittent (ORP-based) 0.08–0.10 mg L−1

Increased COD removal (55%)

Increased removal of all micropollutants

Increased acidogenesis

Wu et al. (2018)
Anionic surfactants AnMBR Air D—intermittent (ORP-based) NM

Removal of surfactant (80%)

No foam

No VFA accumulation

Cheng et al. (2018)
2-Butenal manufacture wastewater EGSB Air D—continuous 0.04–0.10 mg L−1

COD removal (24%)

Increased acidification (21%)

Song et al. (2019)
Textile wastewater (azo dye Direct Black 22) UASB Air D—intermittent (DO monitored daily) 0.04 ± 0.01 mg L−1

No difference in dye removal

Increased aromatic amines removal

Decreased toxicity

Carvalho et al. (2020)
Synthetic pharmaceutical wastewater UASB Air D—intermittent (through feeding line) 0.08 L L−1 d−1

Increased removal of micropollutants (> 50%)

Community not changed

Buarque et al. (2019)
Domestic with pharmaceutical wastewater Anaerobic baffled biofilm-membrane bioreactor (AnBB-MBR) Air D—intermittent (DO monitored) 0.06–0.10 mg L−1

Enhanced adsorption and biodegradation of pharmaceuticals

Ciprofloxacin (78%)

Sulfamethoxazole (89%)

Diclofenac (41%)

Buakaew and Ratanatamskul (2024)
Fresh leachate Sequential reactors (anoxic/micro-aerobic/oxic) Air D—intermittent (relay-controlled DO) < 0.15 mg L−1

Increased acetogenesis

Improved denitrification

Antibiotics removal (50% efficiency)

Wei et al. (2021)
Synthetic wastewater with 2,4-dinitrophenol Membrane-based bubbleless micro-aeration hydrolysis acidification (MBL-MHA) Air D—continuous (through membrane) 0.06–0.60 L L−1 d−1 Degradation of 2,4-dinitrophenol achieving 2 to 3% higher degradation rates compared to bubble aeration method Zhang et al. (2022)
WAS with siloxanes Bach assays O2 D—intermittent (time-based) 1% or 3% (v/v) Enhanced methane production Ortiz-Ardila et al. (2024)

BTEX; benzene, toluene, ethylbenzene, and xylene, WAS; waste activated sludge; MBL-MHA; membrane-based bubbleless micro-aeration coupled with hydrolysis acidification, MABR; membrane-aerated biofilm reactor, UASB; upflow anaerobic sludge blanket, AnMBR; anaerobic membrane bioreactor, EGSB; expanded granular sludge bed, NM; not mentioned, COD; chemical oxygen demand, VFA; volatile fatty acids, DO; dissolved oxygen, OTR; oxygen transfer rate

aDepending on the information available, aeration is expressed either as the aeration rate (volume of O2 per unit of reactor working volume and time), or as the target dissolved oxygen (DO) concentration (mass of O2 per unit of reactor working volume) maintained throughout the experiment