Table 2.
Ingredient composition of experimental diets
| Phase 1 | Phase 2 | |||||
|---|---|---|---|---|---|---|
| Feedstuff, % | Control | Control + 800 TMU/kg β-Mannanase1 |
Control + 100,000 TMU/kg β-Mannanase |
Control | Control + 800 TMU/kg β-Mannanase | Control + 100,000 TMU/kg β-Mannanase |
| Ground corn | 41.90 | 41.892 | 40.65 | 62.63 | 62.622 | 61.38 |
| Soybean meal, dehulled | 28.00 | 28.00 | 28.00 | 32.00 | 32.00 | 32.00 |
| Protein plasma, spray dried | 2.00 | 2.00 | 2.00 | - | - | - |
| Choice white grease | 1.80 | 1.80 | 2.05 | 2.00 | 2.00 | 2.25 |
| Whey powder | 23.00 | 23.00 | 23.00 | - | - | - |
| L-Lys HCl | 0.35 | 0.35 | 0.35 | 0.35 | 0.35 | 0.35 |
| DL-Met | 0.17 | 0.17 | 0.17 | 0.12 | 0.12 | 0.12 |
| L-Thr | 0.08 | 0.08 | 0.08 | 0.10 | 0.10 | 0.10 |
| Dicalcium phosphate | 0.95 | 0.95 | 0.95 | 1.15 | 1.15 | 1.15 |
| Limestone | 0.85 | 0.85 | 0.85 | 0.75 | 0.75 | 0.75 |
| Vitamin-mineral premix2 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 |
| Salt | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 |
| Natupulse TS3 | 0.00 | 0.008 | 1.00 | 0.00 | 0.008 | 1.00 |
| Total | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 | 100.00 |
1β-Mannanase = Natupulse® TS; BASF SE, Ludwigshafen, Germany. The β-mannanase unit TMU is defined as the amount of enzyme that produces reducing carbohydrates having a reducing power corresponding to one µmol mannose from locust bean gum (0.3 g/100 ml buffer solution) in one minute under the assay conditions of 50.0 +/-0.1 °C and pH 3.5.
2Provided the following quantities of vitamins and micro-minerals per kilogram of complete diet: Vitamin A as retinyl acetate, 11,136 IU; vitamin D3 as cholecalciferol, 2,208 IU; vitamin E as DL-alpha tocopheryl acetate, 66 IU; vitamin K as menadione dimethylprimidinol bisulfite, 1.42 mg; thiamin as thiamin mononitrate, 0.24 mg; riboflavin, 6.59 mg; pyridoxine as pyridoxine hydrochloride,0.24 mg; vitamin B12, 0.03 mg; D-pantothenic acid as D-calcium pantothenate, 23.5 mg; niacin, 44.1 mg; folic acid, 1.59 mg; biotin, 0.44 mg; Cu, 20 mg as copper chloride; Fe, 126 mg as ferrous sulfate; I, 1.26 mg as ethylenediamine dihydriodide; Mn, 60.2 mg as manganese hydroxychloride; Se, 0.3 mg as sodium selenite and selenium yeast; and Zn, 125.1 mg as zinc hydroxychloride.
3The 0, 0.008, and 1.0 inclusion levels are equivalent to 0, 100, and 12,500 mg of β-mannanase per kg of complete diet, respectively.