Table 3.

Nutrient composition of experimental diets, as-is basis

	Phase 1			Phase 2
Item	Control	Control + 800 TMU/kg β-Mannanase1	Control + 100,000 TMU/kg β-Mannanase	Control	Control + 800 TMU/kg β-Mannanase	Control + 100,000 TMU/kg β-Mannanase
Gross energy, kcal/kg	3,952	3,986	3,999	4,017	4,029	4,045
Dry matter, %	89.01	89.03	89.07	90.74	90.63	90.76
Ash, %	6.02	6.02	5.48	5.09	5.30	4.93
Crude protein, %	18.39	18.59	18.37	18.70	19.14	19.19
Acid hydrolyzed ether extract, %	4.34	4.00	4.68	5.40	5.45	5.32
Total dietary fiber, %	10.05	9.70	10.00	13.45	13.80	13.80
Insoluble dietary fiber, %	8.50	8.40	8.60	12.50	12.50	12.50
Soluble dietary fiber, %	1.55	1.30	1.40	0.95	1.30	1.30
β-mannanase activity, TMU/kg	< 100	961	116,926	< 100	842	115,013
Indispensable amino acids, %
Arg	1.18	1.19	1.17	1.27	1.30	1.32
His	0.51	0.51	0.50	0.51	0.53	0.54
Ile	0.95	0.95	0.92	0.87	0.90	0.92
Leu	1.78	1.79	1.75	1.71	1.73	1.73
Lys	1.48	1.49	1.46	1.38	1.39	1.39
Met	0.45	0.43	0.43	0.38	0.39	0.37
Phe	0.98	0.99	0.96	1.00	1.02	1.05
Thr	0.91	0.92	0.95	0.82	0.83	0.82
Trp	0.27	0.28	0.27	0.23	0.23	0.23
Val	1.05	1.05	1.03	0.98	0.99	1.01
Dispensable amino acids, %
Ala	1.05	0.99	0.97	0.99	0.99	1.01
Asp	2.24	2.07	1.97	1.99	1.95	2.04
Cys	0.38	0.37	0.32	0.31	0.30	0.31
Glu	3.80	3.57	3.43	3.55	3.54	3.64
Gly	0.82	0.76	0.74	0.81	0.80	0.83
Pro	1.18	1.11	1.10	1.13	1.13	1.13
Ser	0.92	0.88	0.86	0.85	0.85	0.87
Tyr	0.68	0.65	0.61	0.64	0.61	0.65

¹β-Mannanase = Natupulse® TS; BASF SE, Ludwigshafen, Germany. The β-mannanase unit TMU is defined as the amount of enzyme that produces reducing carbohydrates having a reducing power corresponding to one µmol mannose from locust bean gum (0.3 g/100 ml buffer solution) in one minute under the assay conditions of 50.0 +/-0.1 °C and pH 3.5.