Table 6.

Hematological characters of pigs fed experimental diets¹

Item	Reference value2	Control	Control + 800 TMU/kg β-Mannanase3	Control + 100,000 TMU/kg β-Mannanase	SEM	P-value
WBC4, × 103/ul	11.35–28.9	15.05	16.50	17.04	0.65	0.105
RBC4, × 106/ul	5.88–8.19	6.17	6.19	6.22	0.08	0.929
Hemoglobin, g/dl	11.2–14.7	11.13	11.50	11.43	0.15	0.181
Hematocrit, %	32.3–42.6	37.14	38.11	38.02	0.45	0.251
MCV4, fl	47.5–59.2	60.26	61.56	61.36	0.74	0.383
MCH4, pg	16.3–20.6	18.06	18.58	18.44	0.26	0.221
MCHC4, g/dl	33.3–35.8	29.96	30.18	30.08	0.12	0.389
RDW4, %	16.4–32.3	20.85a	19.15b	19.47ab	0.47	0.031
Platelet, × 103/ul	118.9–522.9	388.88	402.46	411.08	22.50	0.780
MPV4, fl	6.8–10.8	10.61a	9.67b	9.94ab	0.23	0.028
Neutrophil, × 103/ul	2.0–10.4	6.92	8.02	8.04	0.59	0.315
Lymphocyte, × 103/ul	5.30–17.9	7.07	7.39	7.75	0.27	0.169
Monocyte, × 103/ul	0.0–3.7	0.55	0.55	0.62	0.03	0.170
Eosinophil, × 103/ul	0.0–1.3	0.31	0.33	0.36	0.04	0.574
Basophils, × 103/ul	0.0–0.4	0.05	0.06	0.06	0.01	0.625
Absolute LUC4, × 103/ul	-	0.13	0.16	0.16	0.01	0.269

^a-bValues within a row lacking a common superscript letter are different (P < 0.05).

¹Data are least square means of at least 8 observations for all treatments.

²Reference Intervals were reported by Iowa State University’s Clinical Pathology Laboratory (2011) and Cooper et al. (2014).

³β-Mannanase = Natupulse® TS; BASF SE, Ludwigshafen, Germany. The β-mannanase unit TMU is defined as the amount of enzyme that produces reducing carbohydrates having a reducing power corresponding to one µmol mannose from locust bean gum (0.3 g/100 ml buffer solution) in one minute under the assay conditions of 50.0 +/-0.1 °C and pH 3.5.

⁴WBC = total white blood cells count; RBC = red blood cell count; MCV = mean corpuscular volume; MCH = mean corpuscular hemoglobin; MCHC = mean corpuscular hemoglobin concentration; RDW = red cell distribution width; MPV = mean platelet volume; LUC = absolute large unstained cells.