FIG. 4.

Autoradiography of CVB3 capsid proteins from peak radioactive fractions, following sucrose gradient separation. Peak fractions following incubation with cell medium only (control) or elution from permissive cells, DAF-Fc, or CAR-Fc were precipitated with trichloroacetic acid and analyzed by Tricine gel electrophoresis. One-hundred-microliter aliquots of these fractions were also dialyzed and assayed for infectivity on permissive cells, and these values (TCID₅₀) are listed below the relevant fractions.