TABLE 3.
Replication of rescued viruses in BALB/c mice inoculated intranasally with 106 EID50 (50 μl)a
| Virusb | Mean virus titer (log10 EID50/ml) ± SD
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Lung
|
Spleen
|
Kidney
|
Brain
|
|||||
| Day 4 p.i | Day 6 p.i | Day 4 p.i | Day 6 p.i | Day 4 p.i | Day 6 p.i | Day 4 p.i | Day 6 p.i | |
| W-DKGX/22 | ||||||||
| W-DKGX/35 | 6.5 ± 0.1 | 6.4 ± 0.4 | 1.9 ± 0.6 | 1.4 ± 0.2 | 1.4 ± 0.3 | 1.9 ± 0.4 | 2.4 ± 1.0 | 5.7 ± 0.2 |
| R-DKGX/22 | ||||||||
| R-DKGX/35 | 6.5 ± 0.1 | 5.9 ± 1.0 | 3.0 ± 0.4 | 2.2 ± 0.7 | 1.8 ± 1.0 | 1.6 ± 0.4 | 2.2 ± 0.4 | 5.0 ± 1.1 |
| GX/22-35PB2 | 4.4 ± 0.4 | 4.7 ± 0.8 | 1.3 ± 0 | 3.3 ± 1.9 | ||||
| GX/35-22PB2 | 5.0 ± 1.6 | 3.8 ± 2.0 | 1.3 ± 0.1d | 2.4 ± 0.4 | ||||
| GX/35-22PB1 | 4.5 ± 2.7 | 6.0 ± 0.4 | 2.0 ± 0.7 | 1.9 ± 0.1 | 1.5 ± 0.4 | 1.3 ± 0.1 | 3.5 ± 0.5 | |
| GX/35-22PA | 5.4 ± 0.4d | 5.5 ± 0.1 | 1.9 ± 0.9 | 3.6 ± 1.3 | ||||
| GX/35-22HA | 6.5 ± 0.3 | 6.5 ± 0.3 | 3.0 ± 0.4 | 2.8 ± 0.1 | 1.6 ± 0.5 | 1.3 ± 0 | 3.0 ± 1.7 | 4.8 ± 0.8 |
| GX/35-22NP | 6.4 ± 0.1 | 6.3 ± 0.5 | 2.8 ± 0.4 | 2.6 ± 0.1 | 2.0 ± 0.7 | 5.1 ± 0.5 | ||
| GX/35-22NA | 5.1 ± 0.5d | 4.5 ± 0.3 | 1.5 ± 0.4c | 1.3 ± 0.1 | 1.2 ± 0.1 | 2.0 ± 1.3 | 3.2 ± 1.8 | |
| GX/35-22M | 5.0 ± 1.5 | 6.1 ± 0.6 | 2.2 ± 0.9 | 2.3 ± 0.7 | 1.2 ± 0.1 | 2.0 ± 1.3 | 1.4 ± 0.3 | 2.8 ± 0.8c |
| GX/35-22NS | 5.5 ± 0.7 | 5.9 ± 0.3 | 1.7 ± 0.9 | 3.6 ± 0.6 | 1.4 ± 0.1 | 4.5 ± 0.7 | ||
| Chimera 1 | ||||||||
| Chimera 2 | 4.5 ± 0.5 | 4.9 ± 0.5 | 1.2 ± 0.1 | 2.5 ± 1.1 | ||||
| Chimera 3 | 2.8 ± 1.8c | 2.0 ± 1.3c | ||||||
| Chimera 4 | 6.2 ± 0.7 | 5.3 ± 1.4 | 1.6 ± 0.5c | 1.3 ± 0.1 | 1.3 ± 0.1 | 2.6 ± 1.8 | 2.6 ± 1.4 | |
| 22/PB2-699K | ||||||||
| 22/PB2-701N | 3.6 ± 0.6 | 2.4 ± 2.0 | 1.6 ± 0.7 | |||||
| 35/PB2-699N | 5.2 ± 0.1d | 4.2 ± 2.6 | 1.5 ± 0.3d | 2.3 ± 0.9 | 1.2 ± 0.1 | 1.3 ± 0.1c | 1.7 ± 0.9c | |
| 35/PB2-701D | 2.5 ± 1.3d | 6.1 ± 1.2 | 1.5 ± 0.2 | |||||
a
Three mice from each group were killed on days 4 and 6 postinoculation (p.i.), and virus in the organs was titrated in eggs. Blank cells indicate no virus detected.
b
Reassortant viruses bearing the PB1, PA, HA, NP, NA, M, and NS genes in the DKGX/22 background were not recovered from any organ of the inoculated mice on day 4 or day 6, and therefore, the data are not shown.
c
P < 0.05 compared with the titers in the corresponding organs of the R-DKGX/35-inoculated mice.
d
P < 0.01 compared with the titers in the corresponding organs of the R-DKGX/35-inoculated mice.