TABLE 1.

Quantification of GM1 recruitment to the VS^a

JurkatLAI effector cell treatment	% Conjugatesb	% VSc	% GM1 polarization	nd
Untreated	42	47	63	129
β-CD treatment	35e	6	7	118

^aJurkat_LAI effector T cells were either untreated or pretreated with 10 mM β-CD for 30 min at 37°C to deplete membrane cholesterol. Washed effector cells were mixed with primary CD4⁺ target T cells for 1 h at 37°C in the presence of L120 to stain CD4. Conjugates were fixed and stained at 4°C with B-Ctx to label GM1 and then permeabilized and stained for HIV Gag. Conjugates were subsequently analyzed by LSCM for VS formation and GM1 polarization.

^bThe percentage of effector Jurkat_LAI cells in conjugates with primary CD4⁺ T cells was calculated by analyzing the number (n) of effector cells shown.

^cVS formation was defined as copolarization to the conjugate interface of CD4 on the target cell and of HIV antigens in the effector cell as described previously (16, 17).

^dn indicates the number of effector cells examined from randomly chosen low-power fields.

^eValue does not differ significantly (P < 0.05) from the control values as calculated using an unpaired two-tailed student t test.