FIG. 2.
Western blot analysis of Pr160gag-pol incorporation in immature p51-RNH cleavage site mutant viruses that contain an inactivated HIV-1 PR (D25A). Immature viruses, previously standardized for p55gag content by densitometry, were ultracentrifuged, lysed, and loaded into each lane. Relative viral content of (A) Pr160gag-pol and (B) Pr55gag were determined by probing separate blots with anti-RT and anti-p24 monoclonal antibodies, respectively, followed by densitometry scanning analysis of ECL-exposed blots under subsaturating conditions.