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Predicted probability for coiled-coil formation in the cytoplasmic domain of M-PMV Env. The amino acid sequences of the membrane-spanning domain and cytoplasmic domain (residues 520 to 586 of Env) for the wild-type and mutant proteins were used for analysis by COILS (40). The output for the region spanning the first 30 amino acids of the cytoplasmic domain is shown.
