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. 2005 Sep;79(18):11864–11872. doi: 10.1128/JVI.79.18.11864-11872.2005

FIG. 7.

FIG. 7.

Analysis of EYFP distribution in nuclear (N) and cytoplasmic (C) fractions of transfected COS-7 cells. COS-7 cells were transfected with plasmids encoding aa121-139-EYFP, VP22-mNLS-EYFP, VP22-dNLS-EYFP, EYFP, NLS-EYFP, dEYFP, NLS-dEYFP, tEYFP, NLS-tEYFP, VP22-mNES-EYFP, VP22-mNES-EYFP (plus LMB treatment), EYFP (plus LMB treatment), NES-EYFP, NES-EYFP (plus LMB treatment), dEYFP (plus LMB treatment), NES-dEYFP, and NES-dEYFP (plus LMB treatment). At 24 h posttransfection, COS-7 cells were fractionated into nuclear and cytoplasmic fractions as described previously (29, 30). Equivalent amounts of each fraction were separated by SDS-polyacrylamide gel electrophoresis on an 8.5% gel, transferred to nitrocellulose, and probed with an EYFP-specific polyclonal antibody.