FIG. 8.

Identification of an NES in VP22. (A) Schematic diagram of the EYFP fusion proteins used to identify the VP22 NES. Thenames of the constructs are shown on the left. (B) COS-7 cells were transiently transfected with plasmids encoding VP22-mNES-EYFP, EYFP, NES-EYFP, dEYFP, and NES-dYEFP, with or without treatment with LMB, and examined live 24 h after transfection by fluorescence microscopy. Each image is representative of the majority of the cells observed in several fields. Magnification, ×40.