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Release of protein E from BHK-21 cells at 37°C (filled circles) and 28°C (empty circles). Cells were transfected with the mutant RNA prM(ΔR88), prM(ΔR88/C79Y), or prM(ΔR88/Y76C), as indicated. The release of protein E into the cell culture supernatants was monitored for 6 days by ELISA.
