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Recombinant expression of UL132. 293T cells were transfected with a plasmid coding for UL132-Myc and cell lysates were prepared 48 h later. Lysates were treated with either PNGase F (pcUL132F) or endoglycosidase H (pcUL132H) or left untreated (pcUL132). Immunoblots were carried out and proteins were visualized using an anti-Myc antibody. Lanes: pcDNA, cell lysate prepared following transfection with plasmid pcDNA3; n.t., lysate from nontransfected 293T cells.
