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. 2005 Sep;79(18):11837–11847. doi: 10.1128/JVI.79.18.11837-11847.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 7. — Replication of UL132 mutant viruses. (A) Fibroblasts were seeded in six-well dishes and infected with the indicated virus strains (multiplicity of infection, 0.1). At the indicated days postinfection, supernatants from the infected cultures were harvested and infectious virus was titrated using indirect immunofluorescence with an antibody directed against the immediate-early antigen 1. Each data point represents the mean of three independent wells. RV-HB5: virus representing AD169; RV-delUL132/AD: recombinant virus lacking ORF UL132; RV-132rev: revertant virus with UL132 reintroduced into RV-HB5. (B) HFF were infected with a multiplicity of infection of 1. At the indicated time points postinfection (days), virus titers in the supernatant of three independently infected cultures (a to c) were determined as described in Materials and Methods. PAN23: recombinant virus PAN; delUL132/PAN: recombinant PAN virus lacking ORF UL132. IU: infectious units.