TABLE 4.

Influence of HIV-1 CRF01_AE RT variants on virus mutation frequencies

RT variant	Mutation(s)a	Drug resistance phenotypeb	Avg relative mutation frequencyc
93JP-NH1	None	None	1
CRF01_AE mt-1	M41L, L210W, T215Y	AZT (mild)	0.80, 0.88, 0.96
CRF01_AE mt-2	Insertiond	3TC (weak)	0.68, 0.92, 1.16
CRF01_AE mt-5	Insertion + AZTe	AZT (high), 3TC (mild), d4T (weak), ddI (weak)	0.76, 0.86, 0.96
CRF01_AE mt-6	Insertiond + AZTe + T69l	AZT (high), 3TC (high), d4T (mild), ddI (mild), ddC (weak)	0.81, 0.87, 0.93
CRF01_AE mt-7	Insertiond + AZTe + T69l and othersf	AZT (high), 3TC (high), d4T (mild), ddI (mild), ddC (weak)	0.59, 0.69, 0.79

^aThe insertion is an 11-amino-acid insertion located between codons 67 and 68 in the β3-β4 loop coding region of the RT gene.

^bSee Table 3, footnote a.

^cEndpoints of 95% confidence intervals and the sample mean are presented. The average number of relative light units for virus replication in the presence of wild-type RT was 6.3 × 10² ± 38.

^dReverse transcriptase variants had an 11-amino-acid insertion between codons 67 and 68 in the β3-β4 loop coding region. The amino acid insertion was NIHGGRDQGPA.

^eReverse transcriptase variants with the zidovudine (AZT) resistance mutations M41L, D67N, K70R, L210W, and T215Y.

^fThe other mutations in reverse transcriptase included D6E, K39E, E43K, T69I, G196E, T200R, and L228R.