FIG. 3.
Immunoprecipitation of radiolabeled gE/gI from cells infected with HSV gE ET domain mutants. R-970 cells were infected with wild-type HSV-1 (F), F-gEβ, and various gE mutants. The cells were labeled with [35S]methionine-cysteine from 6 to 9 h after infection. gE/gI was immunoprecipitated from cell extracts using MAb 3114 or 3063, and proteins were subjected to electrophoresis on polyacrylamide gels. The positions of gE, immature gE (pgE), g, and immature gI (pgI) as well as molecular mass markers of 97, 67, 43, and 14 kDa are shown. W.t., wild type.