FIG. 5.

Replication gE mutants 277 and 348-4. HaCaT cells were infected with F-BAC and gE mutants 277 and 348-4 using 10 PFU/cell. At various times, cells were scraped from plastic dishes into cell culture media, frozen, and sonicated, and HSV titers were determined in plaque assays. The experiments were performed in duplicate, and differences in titers at each time point were small, so that error bars overlapped the symbols and were too small to be discerned. W.t., wild type.