FIG. 7.

Spread of gE mutants 277, 291, 348-4, and 380 in corneal epithelium. BALB/c mice were infected in the cornea with F-BAC; FgEβ; gE mutant 277, 291, 348-4, or 380; or the rescued virus 277R or 348-4R for 2 days. The mice were sacrificed, and their corneas were removed, fixed, permeabilized, and stained with anti-HSV antibodies and fluorescent secondary antibodies. Wide-field fluorescent microscopy was performed on flat-mounted corneas in order to observe thin sections of the corneas amounting to single cells.