FIG. 8.

IgG binding of gE ET domain mutants. HaCaT cells were infected with wild-type HSV-1 (F); F-gEβ; F-gEΔCT; or F-BAC, F-BACgE488, or gE ET domain mutants at 10 PFU/cell for 18 h. The cells were incubated with ⁵¹Cr-labeled, IgG-coated sheep RBC for 2 h at 37°C and then washed, and the radioactivity in cell extracts was counted. The binding of wild-type HSV-1 (F) was arbitrarily set to a value of 100, and the binding levels of F-gEβ and F-ΔCT were compared to that value, while the binding of F-BAC was also arbitrarily set to 100, and the binding levels of F-gEBAC488 and all gE ET domain mutants were compared to that value. W.t., wild type.