TABLE 1.
Characterization of monoclonal antibodies to gB
| MAb | ELISAa | Western blotb
|
Neutralization in:
|
Blocks gB binding to:
|
||||
|---|---|---|---|---|---|---|---|---|
| Denatured | Native | Veroc | C10d | Gro2C | C10 | Gro2C | ||
| SS10 | ++ | + | +++ | + | + | + | + | + |
| SS55 | ++ | − | +++ | + | + | + | + | + |
| SS118 | ++ | − | +++ | + | + | ND | + | ND |
| SS19 | + | + | +++ | − | − | ND | − | ND |
| SS48 | − | − | + | − | − | ND | − | ND |
| DL16 | ++ | − | +++ | − | − | − | − | − |
| DL21 | ++ | − | +++ | − | − | ND | − | ND |
a
Serial dilutions of each antibody were reacted against gB(730t), and binding was determined by ELISA. Antibodies were grouped into good (++), moderate (+), or none (−) binder categories, as a function of their avidity for gB.
b
Antibodies at 1 μg/ml were reacted against Western blots of gB(730t) electrophoresed under native or denaturing conditions. Binding was strong (+++), moderate (+), or not detected according to the exposure time required to develop ECL reactions.
c
Measured as a 50% reduction in plaques on Vero cells.
d
Antibodies were considered positive for neutralization in C10 and Gro2C cells if <25 μg/ml inhibited entry by 50%, measuring β-galactosidase activity 5 hours p.i.