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Use of the position 3358 3′ splice site was restored when the enhancer was inserted into the pT1 deletion. (A) Representative illustrations of the deletion regions of pT1, pT1SD, and pT1SDE. A box represents the inserted enhancer, and the sequence is presented below. (B) Northern blots of total RNA extracted from HeLa cells after transfection of the respective plasmids hybridized to the L1, CMV, and E4 probes. Arrows indicate the positions of the mRNA species produced.
