Abstract
Heat-sensitive Pawn (ts Pawns) of Paramecium aurelia behaved normally when grown at 23° but failed to avoid strong stimuli at 35°. Four of the five ts Pawn lines tested were found to be allelic at a locus known also to carry temperature-independent Pawn mutations. The fifth ts Pawn line complemented all the conditional and unconditional Pawn mutants of the two known loci. This result, together with the patterns of F2 segregation from various crosses, suggested the existence of a third Pawn locus. An additive effect of the unlinked ts Pawn genes was observed. These findings and the significance of ts Pawns as experimental material in behavioral and physiological research are discussed.
Full Text
The Full Text of this article is available as a PDF (1.3 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Kung C., Eckert R. Genetic modification of electric properties in an excitable membrane (paramecium-calcium conductance-electrophysiological measurements-membrane mutant). Proc Natl Acad Sci U S A. 1972 Jan;69(1):93–97. doi: 10.1073/pnas.69.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kung C., Naito Y. Calcium-induced ciliary reversal in the extracted models of "Pawn", a behavioral mutant of Paramecium. Science. 1973 Jan 12;179(4069):195–196. doi: 10.1126/science.179.4069.195. [DOI] [PubMed] [Google Scholar]
- Suzuki D. T., Grigliatti T., Williamson R. Temperature-sensitive mutations in Drosophila melanogaster. VII. A mutation (para-ts) causing reversible adult paralysis. Proc Natl Acad Sci U S A. 1971 May;68(5):890–893. doi: 10.1073/pnas.68.5.890. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wood W. B., Edgar R. S., King J., Lielausis I., Henninger M. Bacteriophage assembly. Fed Proc. 1968 Sep-Oct;27(5):1160–1166. [PubMed] [Google Scholar]