TABLE 3.
Summary of multiplex LAMP-PAS results across all bacteria tested in comparison to established MTB-discrimination multiplex PCR
| Multiplex LAMP-PAS | Multiplex PCR (9) | |||||
|---|---|---|---|---|---|---|
| Positive | Positive | |||||
| MTB (16S) | M. bovis (RD4) | Negative | MTBa (268 bp) | M. bovis (168 bp) | Negative | |
| M. bovis b | 51 | 51 | 0 | 0 | 51 | 0 |
|
M. tuberculosis
b
M. africanum M. microti M. orygis M. caprae |
59 1 1 1 1 |
0 0 0 0 0 |
0 0 0 0 0 |
59 1 1 1 1 |
0 0 0 0 0 |
0 0 0 0 0 |
| NTMc | 0 | 0 | 21 | nad | na | na |
| M. ulcerans | 1 | 0 | 0 | |||
| M. marinum | 1 | 0 | 0 | |||
| M. asiaticum | 1 | 0 | 0 | |||
| Non-mycobacteria | 0 | 0 | 5 | na | na | na |
a
MTB other than M. bovis.
b
Fifty field isolates (M. bovis)/58 clinical isolates (M. tuberculosis) and one reference strain.
c
Nontuberculous mycobacteria (NTM) other than M. ulcerans, M. marinum, and M. asiaticum.
d
na: not applicable.