TABLE 1:
Genetic interactions between mutations in pmo25 and genes in the contractile ring a
| Mutations | 21°C | 25°C | 30°C | 32°C | 36°C |
|---|---|---|---|---|---|
| Wild type | ++++b | ++++ | ++++ | ++++ | ++++ |
| pmo25-2 | +++c | +++ | ++d | ++ | +e |
| pmo25-20 | +++ | +++ | /f | ++ | ++ |
| pmo25-21 | +++ | +++ | / | ++ | + |
| pmo25-35 | +++ | +++ | +++ | ++ | ++ |
| rlc1Δ | + | ++ | / | +++ | ++ |
| rlc1Δ pmo25-2 | + | + | / | + | + |
| rlc1Δ pmo25-20 | / | + | / | ++ | + |
| rlc1Δ pmo25-21 | / | + | / | ++ | ++ |
| rlc1Δ pmo25-35 | + | + | / | ++ | + |
| myo2-ΔIQ1ΔIQ2 | / | ++++ | ++++ | ++++ | ++++ |
| myo2-ΔIQ1ΔIQ2 pmo25-2 | / | +++ | + | + | +/− |
| myo2-S1 | / | +++ | ++ | ++ | + |
| myo2-S1 pmo25-2 | / | ++ | ++ | + | −g |
| myo2-S1 pmo25-21 | / | ++ | ++ | + | − |
| myo2-S2 | / | ++++ | +++ | +++ | +++ |
| myo2-S2 pmo25-2 | / | ++ | + | +/−h | − |
| myo2-S2 pmo25-21 | / | +++ | ++ | + | − |
| myo2-E1 | / | +++ | ++ | ++ | + |
| myo2-E1 pmo25-2 | / | ++ | +/− | − | − |
| cdc12(Δ1-503 or ΔNFH3) | / | +++ | +++ | +++ | +++ |
| cdc12(Δ1-503) pmo25-2 | / | +++ | + | + | +/− |
| cdc12(Δ1-503) pmo25-35 | / | +++ | ++ | + | − |
| rng2-346 | / | +++ | ++ | + | +/− |
| rng2-346 pmo25-2 | / | ++ | +/− | − | − |
| cdc4-8 | / | +++ | ++ | + | − |
| cdc4-8 pmo25-2 | / | ++ | +/− | − | − |
| cdc4-8 pmo25-21 | / | ++ | +/− | − | − |
a
The genetic interactions were evaluated based on growth and color of colonies on YE5S + phloxin B plates or morphology of freshly grown cells on YE5S plates or liquid medium at various temperatures.
b
++++, similar to wt;
c
+++, some cell lysis, cytokinesis and/or polarity defects;
d
++, significant cell lysis, cytokinesis and/or polarity defects;
e
+, massive cell lysis, severe cytokinesis and/or polarity defects with significantly reduced growth;
f
/, not tested;
g
−, inviable or no colonies;
h
+/−, phenotypes between + and −.