Fig. 2.

The anti-inflammatory effect of IAA, G4 PAMAM and G4-IAA in vitro. (a–b) mRNA level of IL-6 (a) and IL-8 (b) in A549 by RT-qPCR. (c–d) mRNA level of IL-1β (c) and IL-8 (d) in BASE-2B by RT-qPCR. Data are presented as means ± SD, with n = 3 experimental replicates per group. One-way ANOVA with Tukey's multiple comparisons test was performed. Groups were compared to each other. ns: p > 0.05; ∗: p < 0.05; ∗∗: p < 0.01; ∗∗∗∗: p < 0.0001. IL-1β: interleukin 1β; IL-6: interleukin 6; IL-8: interleukin 8; TNFα: tumor necrosis factor α; RT-qPCR: real-time polymerase chain reaction; mRNA: messenger ribonucleic acid.