Fig. 3.

Polarization of RAW264.7 induced by G4-IAA. (a) Cell-type composition based on single-cell RNA sequencing analysis. (b) Expression of M1 macrophage-associated genes. (c) Expression of genes associated with M2 macrophage polarization. (d, e) DAPI (blue), CD206 (purple) (d), CD86(purple) (e), G4 PAMAM (green) staining sections of RAW264.7 cells at 1, 2, 4 and 8 hours of co-cultivation of macrophages and IAA-PAMAM complex. (f, g) Relative fluorescence intensity of CD206 (f) and CD86 (g) from quantitative analysis at different time points. Data are presented as means ± SD, with n = 3 experimental replicates per group. One-way ANOVA with Tukey's multiple comparisons test was performed. Adjacent groups were compared. ns: p > 0.05; ∗: p < 0.05; ∗∗: p < 0.01. CD: cluster of differentiation. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)