Fig. 4.

Biocompatibility and distribution of G4-IAA. (a–b) Distribution of G4-IAA in mice (a) or separated organs (b) 12, 24, 48 hours after inhalation (50 mg/kg IAA+2 mg/ml G4 PAMAM) or injection (50 mg/kg IAA+2 mg/ml G4 PAMAM) treatments by fluorescence imaging in vivo. (c–d) Relative intensity of G4-IAA in separated organs 12, 24, 48 hours after inhalation treatment (c) or injection treatment (d) from quantitative analysis of fluorescent images. (e) Representative images of RAW264.7 stained by nucleus (blue), lysosome (red) and G4 PAMAM (green) 2, 4 hours after incubation with G4 PAMAM. Data are presented as means ± SD, with n = 3 experimental (e) or biological (a–d) replicates per group. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)